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Once DNA capture probes were immobilized on optical fibres, we demonstrate their putative application as genosensors in detecting Sus Scrofa DNA in purified samples obtained first using a commercial DNA extraction kit and thereafter using our improved extraction method.
DNA was isolated from 200 mg of feces from each hen using a commercial DNA extraction kit (Tiangen Biotech Corporation, Beijing, China) and quantified using a Qubit 2.0 fluorometer (Invitrogen Corporation, Carlsbad, CA, USA).
For genotyping, DNA was isolated from whole blood using a commercial DNA isolation kit (NucleoSpin, Macherey & Nagel, Düren, Germany).
Stool specimens collected from 700 individuals were filtered, mechanically lysed twice, and incubated overnight with proteinase K prior to DNA extraction using a commercial DNA extraction kit.
One loop of a single P. aeruginosa colony, grown on PIA, was processed for DNA extraction using a commercial DNA isolation kit (Qiagen) according to the instructions of the manufacturer.
Detection was performed by capillary gel electrophoresis, using a commercial DNA sequencing machine.
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We have used a commercial DNA extraction kit for purifying DNA from mature, dark green leaves and from young, pale green folded leaves.
Genomic DNA was isolated using a commercial plant DNA extraction kit (Bioteke, Beijing, China) according to the manufacturer's instructions.
The resulting pellets were collected and the DNA was extracted using a commercial QIAamp DNA mini kit (Qiagen, Valencia, CA, USA) according to the manufacturer's instructions.
DNA was extracted from the sample using a commercial Nucleospin DNA extraction kit according to manufacturer's instructions with some modification (Clontech, CA, USA).
Genomic DNA was prepared from each blood sample by using a commercial genomic DNA extraction kit (TALENT s.r.l., Trieste, Italy).
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using a Genomic dna
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