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Ten or 20 µl of samples were injected on a 5 µM PLRP-S column using a column oven temperature of 60°C and a flow rate of 1.0 ml/min with a gradient of solvent A (0.1% formic acid water) and solvent B (90% acetonitrile with 10 mM ammonium formate) from 90% solvent A and 10% solvent B for 2 min followed by a linear gradient to 95% B in 18 min and a 5 min return to the starting composition.
We maintained the column temperature at 45 °C using a column oven (CTO-10Avp; Shimadzu).
Column temperatures were maintained at 35°C using a column oven (Knauer, Berlin, Germany).
The column temperature was maintained at 45 °C using a column oven (CTO-20A; Shimadzu).
The temperature of the column was kept constant at 30 °C using a column oven LCO 102 from Ecom.
The analytical column was a Cosmosil C18-AR-II (4.6 × 150 mm, 5 μm; Nacalai Tesque, Inc .. We maintained the column temperature at 40 °C using a column oven (CTO-20 AC; Shimadzu).
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A C18 Reverse Phase Alltech analytical column (5 μm, 250 9 4.6 mm) was used and maintained at 30 °C in a column oven.
A column oven was used and set to 40°C.
An Alltech C18 Reverse Phase (5 μm, 250 × 4.6 mm) analytical column was used and maintained at 30°C in a column oven.
Analytical separation was performed with a column oven at 40°C using a Zorbax SB-C18 column with a Zorbax SB-C8 pre-column at a flow rate of 0.2 ml/min and a gradient elution.
A Waters (Milford, MA, USA) ACQUITY Ultra Performance LC (UPLC) system, consisting of a degasser, a binary gradient pump, an autosampler (at 10 °C) and a column oven (at 40 °C), was used.
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