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All patients were tested for G6PD deficiency at enrolment using a colorimetric test (Sigma Diagnostics, Poole, UK).
Cell proliferation was measured using a colorimetric test based on the capability of metabolic active cells to cleavage the yellow tetrazolium salt (XTT) to form a soluble orange formazan dye tetrazolium salt (Cell Proliferation Kit II XTT, Roche).
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In addition to a specific quantitative assay for serum/plasma β-hydroxybutyrate (Stanbio Laboratory, Boerne, TX, USA), a semi-qualitative test using a colorimetric assay was also available for assessment of serum and urine ketones (Acetest®, Bayer Diagnostics, now part of Siemens Healthcare Diagnostics, Deerfield, IL, USA).
Next, extracts that showed significant cytotoxicity to Panc-1 cells (> 80% cell death at a testing concentration of 100 μg/mL) in the PC biosensor assay were tested using a colorimetric MTT assay on two additional pancreatic cell lines (Mia-Paca2, and Capan-1).
To examine the time course of choline production from lysoPAF hydrolysed by purified FLAG-tagged Gαq subunit, the enzyme was incubated with 0.15 mM lysoPAF for 0 6 h and activities were tested using a colorimetric assay.
A method using a colorimetric carbon dioxide detector has been tested as a means of decreasing the risk of inadvertent airway placement of gastric tubes in adults, but to date, a similar study has not been accomplished in pediatric patients.
All animals killed at day 12 were also tested for glycaemic blood levels, by using a colorimetric glucose oxidase assay (One-Touch; Lifescan).
A group of animals not behaviorally tested was assayed for tissue and serum Cr levels using a colorimetric Cr assay (Cr Assay Kit, Caymen Chemical, Ann Arbor, MI).
Mg2+ was measured using a colorimetric technique, and phosphate was measured using an ultraviolet technique.
Total flavonoids content was determined using a colorimetric method as described by Heimler et al. [26].
DPPH was determined using a colorimetric method as described by Chen and Ho [29].
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