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TGase activity was measured using a colorimetric procedure in which N-α-carbobenzoxyl-glutaminyl-glycine (N-CBZ-Gln-Gly) (Sigma, Shanghai, China) was used as the substrate [ 6].
Within the ProImmun trial general oxidative stress levels and antioxidative capacity are measured in peripheral blood samples using a colorimetric procedure (FORMplus, Incomat, Glashütten, Germany).
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The activity of LDP can be determined using a colorimetric assay procedure that employs Ser-Met as the substrate in a sodium cacodylate or acetate buffer with pH 5.5.
A 30-μl sample of blood was taken from a fingertip and immediately analysed using a colorimetric assay procedure (Reflotron, Boehringer Mannheim, Germany).
ATP hydrolytic activity of pig gastric microsomes was quantitated as orthophosphate release from substrate ATP using a colorimetric malachite green procedure [ 18].
Serum alanine transaminase (ALT) and aspartate transaminase (AST), creatinine (Cr), cholesterol (Cho), triglyceride (TG), and glucose (Glu) levels were measured following standard procedures using a colorimetric analyzer (Dri‐Chem 3000).
The work presented here describes modified expression techniques that allow for the production of soluble, active RFAP synthases in E. coli along with the procedure for measuring RFAP synthase activity using a colorimetric assay.
Free glycerol levels present in the medium were determined using a colorimetric kit (Free Glycerol reagent; Sigma-Aldrich) and normalized to cell density.
The catalytic activity of PARP2 measurement was carried out using a colorimetric assay which can detect the incorporation of biotinylated-NAD+ into PAR.
Tissue viability was evaluated after 72 h using a colorimetric cell proliferation assay.
Mg2+ was measured using a colorimetric technique, and phosphate was measured using an ultraviolet technique.
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