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Serum glucose was measured by the glucose oxidase method using a colorimetric meter (Glucose Assay Kit).
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Free glycerol levels present in the medium were determined using a colorimetric kit (Free Glycerol reagent; Sigma-Aldrich) and normalized to cell density.
The catalytic activity of PARP2 measurement was carried out using a colorimetric assay which can detect the incorporation of biotinylated-NAD+ into PAR.
Tissue viability was evaluated after 72 h using a colorimetric cell proliferation assay.
Mg2+ was measured using a colorimetric technique, and phosphate was measured using an ultraviolet technique.
Total flavonoids content was determined using a colorimetric method as described by Heimler et al. [26].
DPPH was determined using a colorimetric method as described by Chen and Ho [29].
Condensed tannin content was determined using a colorimetric method as described by Broadhurst and Jones [27].
Urinary Crt was measured using a colorimetric activity assay (R&D Systems, UK).
Catalase was measured using a colorimetric kit (Bio-diagnostic, Egypt) according to Aebi (1984 ).
Iron content was estimated using a colorimetric ferrozine assay [19].
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