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Catalase was measured using a colorimetric kit (Bio-diagnostic, Egypt) according to Aebi (1984 ).
Hydrogen peroxide (H2O2) was measured using a colorimetric kit (Bio-diagnostic, Egypt) as previously described (Fossati et al. 1980).
Finally, lactate levels were measured using a colorimetric kit (Abcam).
Triglyceride quantification in muscle and feces was performed using a colorimetric kit from Biovision (Milpitas, CA, USA).
Insulin was assayed in duplicate using a specific human insulin ELISA kit from Linco (St. Charles, MO) and FFA using a colorimetric kit (NEFA-HR [ 2], Wako Diagnostics).
Total nitric oxide metabolites concentration was evaluated in sample columns effluent by using a colorimetric kit assay according to the manufacturer instructions (R & D Systems).
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The GPX activity was measured as described previously [ 18] using a colorimetric assay kit (Cellular GPX Assay kit; Calbiochem, San Diego, CA, USA), where GPX activity is derived from quantification of NADPH oxidation (absorbance at 340 nm) in a solution also containing reduced glutathione, glutathione reductase, and tert-butyl hydroperoxide to initiate the reaction.
Glucose was determined using a colorimetric commercial kit (SigmaChemical Co .. Plasma insulin concentrations were measured in duplicate by a rat/mouse insulin ELISA kit, using rat insulin as the standard (Linco Research, St. Charles, MO) according to the manufacturer's instructions.
The assay was carried out by using a colorimetric, commercial kit (Calbiochem-Novabiochem Corporation, USA) according to the manufacturer instructions.
Lactate measurement assay: Lactate concentrations in the culture media were measured using a colorimetric assay kit (SUNY at Buffalo).
We have also investigated this phenomenon by measuring the activity of caspases by using a colorimetric caspases kit that measures the activity of caspases-3 in the samples.
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