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After incubation, the number of colonies was tracked using a colony counter.
After 21 days, cells were stained with 0.005% crystal violet solution and foci counted using a colony counter.
Colonies were counted using a colony counter (BioLogics, Manassas, VA, USA).
> -wrap-foot> After staining the tissue culture dishes (20 mm), colonies were counted using a colony counter.
Only the colonies with 50 or more cells were counted as survivors by using a colony counter (Imaging Products).
The inoculated plates were incubated at 37°C for 24 hours and the colonies were counted using a colony counter.
Similar(53)
From each dilution, 0.1 ml was spread on an MCA plate and incubated for 24 h at 37°C, after which the number of pink colonies were counted using a manual colony counter.
The following morning, the number of colony-forming units (CFU /ml was counted using a manual colony counter.
Colony-forming units were quantified after 2 days (YEPD plates) or 3 days (galactose plates) of growth using a Scanalyser Colony Counter (LemnaTec, Wuerselen, Germany).
After 21 days, cells were stained with 0.05 % crystal violet solution (Thermo Scientific #88101) and foci were counted using a ColCount colony counter (Oxford Optronix, UK).
Briefly, a CASY cell counter (Schärfe systems) was used to measure the cell counts, 500 cells were plated on full media (YEPD) agar plates and CFUs were quantified after 2 days of growth using a Scanalyzer Colony Counter (LemnaTec).
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