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The passive sampler yielded highly reproducible Rs values and its PAH uptake was shown to be enhanced by using a collection substrate modified with a gas adsorbing coating (Tenax®) which was exclusively addressed being uptake from the gaseous phase.
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Previously reported analyses of HDV ribozyme cleavage using a collection of small substrates revealed that the identity of the base pairs involving the pairing of substrate position +4 with the ribozyme significantly influenced the activity level [47].
The c-plain sapphire substrate was set at a fixed distance (2.5 cm) downstream the particles as a collection substrate.
The experiments were carried out using a plain aluminum collection substrate in the impactor.
Even so, the observed substrate specificity preferences found for positions −1 to −4 adjacent to the cleavage site using a small substrate agree in part with the results observed for the collection of SOFA-HDV ribozymes targeting the HCV strands.
HRP labelling was visualised using a NovaRed substrate kit (Vector Laboratories, Burlingame, CA, USA).
Xase and prothrombinase activity were assayed using a chromogenic substrate.
As a consequence, the new display is 90% lighter than comparable ones using a glass substrate.
Colour was developed using a Fast Blue substrate.
Also, consider using a different substrate.
A 25-mm glass-fiber filter (type A/E; Pall Life Science, Ann Arbor, MI) was used as the collection substrate.
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