Sentence examples for using a coding sequence from inspiring English sources

We compared the pattern of expression of all Gata4 transcripts using a coding sequence (cds) riboprobe to exon 1-specific Gata4 transcripts using E1a and E1b-specific riboprobes.

These blocks were defined by McLysaught et al., using a coding sequence cutoff value of e = 10-7.

Using a coding sequence polymorphism we show that in most cases (seven out of nine) tumour expression is predominantly driven from one AURKB allele.

This apparent 5′-UTR extension of the TFC6 mRNA was verified by RT-PCR analysis using a coding sequence primer pair and two different primer pairs to amplify sequences upstream of the normal TFC6 transcription start sites.

The gaps in the ortholog protein alignments were used to produce a coding sequence alignment using PAL2NAL (Suyama et al. 2006).

This was carried out in CHO cells as described in Clarkson et al (1999) using a luciferase coding sequence downstream of an egr-1 promoter fragment comprising 624 bp 5′ of the initiation codon.

So far, all studies regarding the function of the PEG10 proteins used expression constructs with a coding sequence starting with a predicted AUG start codon in exon 2. This AUG with its surrounding sequence CCCAACAUGA adheres closely to the Kozak sequence, GCCACCAUGG, considered to be optimal as a translation initiation site (TIS) [38].

In case a library of promoters is used, constructs obtained would contain a coding sequence under control of different promoters.

Then, the protein sequence alignment was converted to a coding sequence alignment using the "Bio::Align::Utilities" module in the BioPerl package (http://www.bioperl.org/).

Then, the protein alignment was transformed into a coding sequence alignment using the "Bio::Align::Utilities" module of the BioPerl package (http://www.bioperl.org/, last accessed February 6 , 2013.

The tBLASTn output was filtered by top bit score and e value per query, then the query hit was used to score conservation, defined as a coding sequence (CDS) having >60% sequence identity over >70% of the length of the reference CDS.