Sentence examples for using a coding primer from inspiring English sources

Mouse Grb2 (residues 56-152) cDNA was amplified using a coding primer incorporating a SphI site and a non-coding primer which incorporated the linker sequence, mouse LAT residues 171-178 and a SacI site.

A CFP fragment was created by PCR amplification of mCFP residues 1-228 using a coding primer incorporating a HindIII site, a kozak sequence, and the 13 N-terminal residues of mouse Lck and a non-coding primer incorporating a SphI restriction site.

This apparent 5′-UTR extension of the TFC6 mRNA was verified by RT-PCR analysis using a coding sequence primer pair and two different primer pairs to amplify sequences upstream of the normal TFC6 transcription start sites.

All AFLP markers were named using a code for each primer combination, followed by sequential numbers for scored bands e.g. p3b1.

Furthermore, Binladen et al. [21] used a primer coding technique and produced 256 tagged primers for use in multiple parallel sequencing, allowing 256 samples to be sequenced in a single run.

p62 delta PB1 4xLIR was generated as follows: pETDuet-6xHis-TEV-mCherry-p62 delta PB1 was used as template for a PCR reaction using a forward primer with an overhang coding for the GSGSSGGDDDWTHLSS amino acid sequence.

The cDNA was later amplified for 26 cycles using a specific primer set for the coding region of the transposase gene (as forward primer: 5′-TTTTTACCAACCCCATTGGA-3′ and reverse primer R1) and the 16S gene (as forward primer: 5′-ATCGGGGAGTAGCTTGCTAC-3′ and reverse primer: 5′-CTAGAGATCGTCGCCTAGGTA-3′) belonging to Regiella.

The coding sequence was amplified using a sense primer comprising a NheI site (5'-ATTAGCTAGCGCCACCATGGAGGGATTCGACGGTTCA-3') and an antisense primer containing a EcoRI site (5'-TGGAATTCTTAGGAATGAGCTACTGCATCTTCTACCTG-3').

To determine whether the bcl2 splice acceptor in the Ds insertion trapped the dhx37 transcripts, we prepared total RNA from dhx37 mutant, sibling, and wild-type embryos at 3 d after fertilization and performed reverse-transcription PCR (RT-PCR) using a primer in the first coding exon (exon 2) of the dhx37 gene and a primer in the mCherry coding sequence.

To this purpose, we performed PCR amplifications on DNA from the monochromosomal somatic hybrids, using a primer in the WASH genes coding region and the other within DDX11L genes.

In the MSD detector, the detector temperature of the transfer line (full scan) was set at 280°C Partial 16S rRNA gene sequences were amplified from replicates of sediment samples using the coded-primer (tag) approach to multiplex pyrosequencing.