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C-reactive protein (CRP) was measured using a clinical chemistry analyser.
Creatinine was measured using a clinical chemistry automated analyzer (Olympus AU400; Olympus America Inc., Melville, NY, USA) using reagents, calibrators, and standard operating procedures as specified by the manufacturer.
Using a clinical chemistry and mass spectrometric approach we analyzed metabolites related to arginine metabolism from plasma samples of polymicrobial infected mice at 6 hours and 24 hours post sepsis induction in comparison with healthy animals.
Serum levels of glucose, cholesterol, triglycerides, high-density lipoprotein-cholesterol (HDL-C), aspartate aminotransferase (AST), alanine aminotransferase (ALT) and alkaline phosphatase (ALP), as well as total bilirubin and direct bilirubin were analyzed using a Clinical Chemistry System from Random Access Diagnostics.
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All biochemical assays were performed using a clinical automatic chemistry analyzer (Type 7170A, Hitachi, Japan).
Standard blood chemistry was determined for eligibility testing after an overnight fast using EDTA plasma from the peripheral venous blood using a routine clinical chemistry analyser (Abbott Diagnostics).
Urine creatinine concentrations were measured using a Dimension clinical chemistry system with a Flex reagent cartridge in an enzymatic assay (Siemens Dimension Vista 1500; Siemens Medical Solutions USA, Inc., Malvern, PA, USA).
Plasma was analysed for the following parameters: total protein, total bilirubin, creatine kinase activity, aspartate aminotransferase activity, cholesterol, creatinine, albumin, alkaline phosphatase activity, gamma-glutamyl transferase activity, triglycerides and glutamate dehydrogenase using a Konelab clinical chemistry analyzer (Thermo Scientific, Waltham, MA).
Hematology analyses were performed using an ADVIA 2120i (Siemens), and biochemistry analyses including C-reactive protein (CRP) were performed using an automated clinical chemistry analyzer (ADVIA 1800 Chemistry System, Siemens) following the manufacturer's instructions.
The amount of plasma lipoproteins in the serum or the FPLC fractions was detected using an automated clinical chemistry analyzer (Alpha Wassermann, USA) or a manual assay according to the manufacturers instruction (Wako, USA).
Serum hs-CRP levels were measured with a high sensitive immunoturbidimetric assay (Diasis Diagnostic System) using an automated clinical chemistry analyzer.
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