Sentence examples for using a cleavage enzyme from inspiring English sources

The circular MIP probes were then linearized using a cleavage enzyme and amplified by PCR.

Trypsin was used as a cleavage enzyme with a maximum of 2 missed cleavages was allowed.

Trypsin was used as cleavage enzyme and one missed cleavage allowed.

Although initial in vitro and even in vivo studies have been carried out using DNA cleavage enzymes targeting various viruses, many questions still remain concerning the feasibility of these strategies as they transition into preclinical research.

We have developed a general approach for global identification of proteolytic cleavage sites using an engineered enzyme to selectively biotinylate free protein N termini for positive enrichment of corresponding N-terminal peptides.

The time course of the cleavage of the wild-type enzyme in the presence and absence of Et-KuQ has been followed using a suicide cleavage substrate.

This task is achieved by using an enzyme cascade reaction that combines a cleavage DNAzyme (17E) and a ligation DNAzyme (E47).

Interestingly cobalt, but not magnesium, calcium or manganese, supported cleavage of the topoisomerase IIβ enzyme using a metal affinity cleavage assay based on Fenton chemistry [42] (unpublished data).

Caspase 3 was assayed using an enzyme activity kit (BIOMOL) according to manufacturer's instructions, by measuring the cleavage of a colorimetric substrate, Ac-DEVD-pNA.

The third wave assay uses Cleavage Enzymes to recognize and cleave specific structures formed by the addition of two oligonucleotides (an Invader Oliga and Primary Probe) to the nucleic acid.

A fundamental problem quantifying site-specific protein phosphorylation is that phosphorylation sites may be located near lysine and arginines such that short, polar peptides may be produced when using trypsin as the cleavage enzyme.