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For example, the common practice of using a clean blade to cut the cord may be through messages to prevent tetanus.
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Wait until flowers are withered or dropping petals and use a clean blade or secateurs to snip the stem back to the first cluster of 5 leaves.
The small tubers were wounded lightly before infiltration using a clean razor blade to facilitate the entrance of Agrobacteria carrying the TRV plasmids.
Biopsies were minced on a clean microscope slide using a clean scalpel blade.
The 12 kb was visualized using blue light and extracted using a clean razor blade.
The gel was then reassembled and the identified size selected band was excised using a clean scalpel blade.
Electrophoresed proteins were detected using the protocol of Shevchenko et al. and bands of interest excised using a clean razor blade.
The gel area containing 20 250 kDa proteins was cut into four slices using a clean razor blade, and each slice was transferred into a new tube.
The area of interest on the 5-μm slide section was selectively macrodissected using a clean razor blade and transferred to a 1.5-ml microfuge tube.
Spots of interest were excised using a clean razor blade, and amino acid sequencing was performed essentially according to the procedure reported by Tao and Kasarda [ 35], but using an Applied Biosystems Procise 492 sequencer.
To summarize, fragments of interest were recovered using a clean razor blade and extracted from the gel matrix by boiling in 200 μL of buffer (10 mM Tris/1 mM EDTA/1% SDS (w/v), pH 8.0) for 15 min. After overnight precipitation at -80°C, the eluted DNA was reamplified using the same primers and PCR conditions as the ones used in the DD-PCR step.
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CEO of Professional Science Editing for Scientists @ prosciediting.com