Sentence examples for using a classical pcr from inspiring English sources

Detection of APV in nestling feces, blow fly larvae and Dermanyssus mites was conducted by using a classical PCR assay [36].

This yeast strain DFY046 (Mat a leu2-3,112 trp1-1 ura3-1 can1-100 ade2-1 his3-11,15 RAD5 bar1DELTA::KMX) is derived from S288c background in which the BAR1 gene encoding a yeast protease was replaced by KMX using a classical PCR deletion technique [5].

The first step was carried out on 50 ng of dog genomic DNA using a classical PCR protocol and a touchdown program of 61°C to 51°C.

The CD cohort and the trio control cohort were typed for HLA-DQA1 and -DQB1 genes using a classical PCR-SSCP/heteroduplex method in an official HLA typing laboratory as described elsewhere [12], [15].

Expressive vocabulary is assessed using a classical naming task.

A risk adjustment model was developed using a classical single-level regression.

Integrity and quality of mRNA (contamination assessment of genomic DNA) were checked with the A260/A280 ratio with Nanodrop (Thermo Scientific) and on agarose with a classical PCR using a reference gene (see primers below).

The RT-PCR of Vázquez-Morón et al. [ 30] uses a nested classical PCR system with degenerate primers and a gel-based DNA detection system.

Operative technique – We used a classical dorsal approach.

All seven used a classical delay conditioning design during acquisition.

We first used a classical Ca2+-dependent apoptosis inducer, Tg.