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The exonuclease protocol for generating inserts is therefore able to generate many times more constructs than would be possible using a classic PCR cloning strategy, but requires the extra step of colony picking to isolate individual clones.
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Using a classic codesign approach.
BAL was performed using a classic protocol.
These conditions can be illustrated using a classic example.
To validate each new set of primers, a classic PCR was performed, followed by qPCR with SYBR Green.
First, I used a classic Timbuk2 messenger.
Over a 2-week span in February, researchers used both the ReEBOV test and a classic PCR-based test on 106 patients who showed up at two clinics in Sierra Leone with suspected Ebola symptoms.
All samples of MDCK and MDCK-Ras cells tested negative for mycoplasma infection by PCR using the Venor®GeM Classic PCR-Kit (Minerva Biolabs; #11-1050) according to the manufacturer's instructions.
Real-time PCR was performed using a TaqMan PCR kit and an Applied Biosystems 7300 Sequence Detection System (Applied Biosystems).
The cDNA fragments were purified using a QIAquick PCR extraction kit following the manufacturer's instructions.
Env was amplified using a nested PCR.
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