Sentence examples for using a chromogenic substrate from inspiring English sources

Compounds were screened for inhibition of HGFA activity in a kinetic enzyme assay using a chromogenic substrate.

At end-point, G-quadruplexes are forced to fold from PCR-generated duplex DNA and then are used to colorimetrically report the successful occurrence of PCR by assaying their peroxidase activity using a chromogenic substrate.

LE activity was measured using a chromogenic substrate: from comparison of the different levels of inhibition, it was deduced that a crucial role in inhibition might be played by a galloyl moiety or hydroxyl group at C3, three hydroxyl groups at B ring, one hydroxyl group at C4′, and a 2,3-double bond.

The bands were visualized using a chromogenic substrate for alkaline phosphatase.

Osteopontin (OPN) was detected by using a chromogenic substrate for the secondary antibody according to the kit manufacturer's instructions.

The designed molecules with the best scoring functions (calculated binding energies) were synthesized and their thrombin inhibitory activity evaluated experimentally in vitro using a chromogenic substrate in a buffer system and using a thrombin generation test in isolated plasma and in vivo using the newly developed model of hemodilution-induced hypercoagulation in rats.

Bound antibodies against PA were detected by using a chromogenic peroxidase substrate (tetramethylbenzidine).

IHC analysis was performed on 4 μm sections in the Ventana Benchmark system (Ventana Medical Systems Inc., Tucson, AZ, USA) using prediluted antibodies to the ER (anti-ER, clone 6F11; Ventana) or to the PR (anti-PR, clone 16; Ventana) as previously described [ 11]. 3,3'-Diaminobenzidine (DAB) was used as a chromogenic substrate, and the slides were counterstained using haematoxylin.

MOM kit BMK-22022, Vector laboratories) was used to block nonspecific binding of secondary antibodies and 3.3'-diaminobenzidine (DAB) was used as a chromogenic substrate.

Proteolytic predigestion using proteinase K was performed for 6 min. 3-Amino 9-ethyl carbazole was used as a chromogenic substrate and Mayer haemalum as a counterstain.

Diaminobenzidine (Menarini) was used as a chromogenic substrate.