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The formed phosphate could be quantified by using a chromogenic reaction allowing spectrophotometric detection of the degree of conversion.
After incubation and washing, bound antibody was detected using a specific anti-IgG antibody linked to an enzyme and visualised using a chromogenic reaction.
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Enzymatic properties of cholesterol oxidase from Rhodococcus strains (some of which named formerly as Nocardid) are particularly suitable for use in the analytical determination of cholesterol, in which the hydrogen peroxide formed is used in a chromogenic reaction catalyzed by horseradish peroxidase [ 6].
After stringent washing, streptavidin-conjugated alkaline phosphatase was added and a non-radioactive reaction performed using a chromogenic substrate.
Total coliform and E. coli analyses were performed using a chromogenic medium.
Total coliform and E. coli analyses were performed using a chromogenic medium (Colilert-IDEXX®; IDEXX Laboratories, Inc., ME, USA).
The bands were visualized using a chromogenic substrate for alkaline phosphatase.
Using a chromogenic assay, we assessed activity of PL in psoriasis.
Osteopontin (OPN) was detected by using a chromogenic substrate for the secondary antibody according to the kit manufacturer's instructions.
Elastase activity was thereafter measured using a chromogenic assay.
Anti-Xa levels were measured photometrically using a chromogenic technique (ACL top, Instrumentation Laboratory).
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