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Bound antibodies against PA were detected by using a chromogenic peroxidase substrate (tetramethylbenzidine).
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First, as CISH uses a chromogenic peroxidase reaction to reveal amplification signals, samples can be assessed with a standard light microscope, allowing simultaneous viewing of staining and histopathology.
At end-point, G-quadruplexes are forced to fold from PCR-generated duplex DNA and then are used to colorimetrically report the successful occurrence of PCR by assaying their peroxidase activity using a chromogenic substrate.
Horseradish peroxidase activity was detected using a chromogenic substance, o-phenylenediamine tetrahydrochloride (Sigma) in citrate-phosphate buffer (pH 5.4) and H2O2 as 1 µl/ml.
Total coliform and E. coli analyses were performed using a chromogenic medium.
Total coliform and E. coli analyses were performed using a chromogenic medium (Colilert-IDEXX®; IDEXX Laboratories, Inc., ME, USA).
The bands were visualized using a chromogenic substrate for alkaline phosphatase.
Using a chromogenic assay, we assessed activity of PL in psoriasis.
Osteopontin (OPN) was detected by using a chromogenic substrate for the secondary antibody according to the kit manufacturer's instructions.
Elastase activity was thereafter measured using a chromogenic assay.
Anti-Xa levels were measured photometrically using a chromogenic technique (ACL top, Instrumentation Laboratory).
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