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The antithrombin III activity was quantitated using a chromogenic activity assay (Stachrome ATIII; Diagnostica Stago).
Antithrombin III activity was quantitated using a chromogenic activity assay (Stachrome ATIII; Diagnostica Stago).
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Using a chromogenic assay, we assessed activity of PL in psoriasis.
Explicitly, when prothrombinase is assembled with a factor Va molecule possessing a heavy chain that is truncated at the acidic hirudin-like COOH-terminal region, a discrepancy is observed between the activity of factor Va measured by the clotting assay and the activity of the cofactor measured in an assay using a chromogenic substrate to assess thrombin activity.
ABE-II is exposed following cleavage of meizothrombin at Arg and release of fragment 2. We show that the catalytic activity of prothrombinase when assembled with the mutant cofactor molecules is only moderately affected by the mutations, when the activity of factor Va is measured in an assay using a chromogenic substrate to assess thrombin activity.
At end-point, G-quadruplexes are forced to fold from PCR-generated duplex DNA and then are used to colorimetrically report the successful occurrence of PCR by assaying their peroxidase activity using a chromogenic substrate.
The antibody conjugated with human tissue factor (MECA32-TF) was characterized for its tissue factor activity using a chromogenic substrate assay [ 16], and for binding to mouse PLVAP using an ELISA assay (Additional file 2: Supplementary Methods).
Moreover, ETHE1 does not display β-lactamase activity either using a chromogenic cephalosporin (nitrocefin), a penicillin (penicillin G) or a carbapenem (meropenem) as substrates under our standard β-lactamase assay conditions (32).
LE activity was measured using a chromogenic substrate: from comparison of the different levels of inhibition, it was deduced that a crucial role in inhibition might be played by a galloyl moiety or hydroxyl group at C3, three hydroxyl groups at B ring, one hydroxyl group at C4′, and a 2,3-double bond.
Horseradish peroxidase activity was detected using a chromogenic substance, o-phenylenediamine tetrahydrochloride (Sigma) in citrate-phosphate buffer (pH 5.4) and H2O2 as 1 µl/ml.
Antifactor Xa activity was determined using a chromogenic substrate (COACUTE®, Chromogenix AB, Mölndal, Sweden).
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