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This method measures amylolytic activity using a chromagenic substrate, 2-chloro-p-nitrophenol, linked to maltotriose.
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Immunocytochemistry was accomplished using rabbit anti-mouse BCL2 polyclonal antibody (Upstate, Waltham, MA), or an antibody recognizing active caspase-3 (Cell Signaling, Beverly, MA) using a Vectastain ABC elite kit (Vector Labs, Burlingame, CA), and 3, 3'-diaminobenzidine (Vector Labs) as a chromagenic substrate with hematoxylin counterstaining.
Immunoreactivity was detected with VECSTATIN ABC Elite kit (Vector Labs, Burlingame, CA) with DAB (Vector Labs) as a chromagenic substrate with hematoxylin counter-staining.
As a consequence, the new display is 90% lighter than comparable ones using a glass substrate.
Colour was developed using a Fast Blue substrate.
Also, consider using a different substrate.
With the ability to detect alterations in heat using a substrate underlay, the patch changes color when it senses an issue.
The membranes were developed using an ECL substrate.
The display, demonstrated at the University of Cincinatti, uses a different substrate for the pigment used to create black areas on the display: oil.
In this study, the substrate specificity of 3CLpro was profiled using a library of protein substrates.
The percentage of positive virus wells per dilution was detected using True Blue chromagenic peroxidase substrate (KPL, Gaithersburg, MD), which selectively stained only the positive wells.
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