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A TaqMan quantitative real-time reverse transcription PCR (qRT-PCR) was performed on all animal serum samples by using a certified real-time RT-PCR kit for clinical diagnosis (SFDA registration no. 340166, China).
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Real-time PCR was performed using a Bio-Rad CFX96TM real-time PCR system and SYBR real-time Master Mix TIANGENN).
Quantitative real-time RT-PCR was performed using a Stratagene MX3000P Real-Time Cycler.
SYBR Green real time PCR was performed using a Biorad CFX96 Real-Time PCR detection system.
Real-time PCR was performed using an Applied Biosystems 7500 Real-time PCR System.
Real-time PCR was done using an Applied Biosystems 7500 real-time PCR system.
Real-time PCR for microRNA was performed using an Applied Biosystems 7500 Real-Time PCR System.
Real-time PCR amplification and analysis were carried out using an Applied Biosystems 7500 real-time PCR system (Applied Biosystems, Foster City, CA).
qRT-PCR (quantitative real-time-PCR) was performed using an ABI 7900 real-time PCR instrument.
Comparative real-time PCR was performed using a Stratagene Mx3000 P real-time instrument in a 96 well format.
Real-time PCR was performed using a 7300 Real-Time PCR System (Applied Biosystems).
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