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To the best of our knowledge, this is the largest surveillance study to assess glycaemic control in Brazil using a certified method to measure HbA1c.
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We used a certified method to analyse glycated haemoglobin.
To validate the precision and the sensitivity of our method, we used the standard addition method using a certified Cr VI) standard solution (Fluka, Milan, Italy).
In order to assess the accuracy of the Pittsburgh Green II-based method for quantifying HSA in human serum, we compared this method with the BCG method by using a certified reference material human serum standard (ERM-DA470k/IFCC) whose HSA concentration was determined to be 36.9 ± 1.2 g/L as the average of many analytical methods.
This study illustrates on the benefit of using a certified reference material to validate methods and procedures involving digital PCR and the risk of relying on data generated by a software which makes assumptions on the value of one or more experimental variables.
The method was validated using a Certified Reference Material (C.R.M. 1086a; Wear Metals in Oil, N.I.S.T., Gaithersburg, MA) and applied to five different used industrial oils.
The accuracy of the method was verified using a certified reference material (GBW 07302 Stream Sediment) by aqueous standard calibration curves.
The digestion procedure was proposed from the conclusions of a fractionated factorial design, and the precision and accuracy of the method was verified using a certified reference sediment RTC008-0500).
The accuracy of method was also checked for biological samples using a certified reference material DORM-3 (Fish ProTraceCertified Reference Metalsal for Trace Metals).
The method was validated according to international guidelines and using a certified reference material.
Using the optimized extraction conditions, the method was validated in terms of accuracy, and precision (within-a-day and among-days), using a certified reference material (CRM 115) as well as spiked cattle feedingstuffs at different concentration levels.
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