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Protein binding in mice serum was measured by an ultrafiltration method described by Craig and Suh [31] for cefditoren and ceftriaxone concentrations of 4 µg/ml and 64 µg/ml, respectively, by using a centrifugal system device (Centrifree®, Amicon Bioseparations, Millipore, Tullagreen, Ireland).
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The particle size distributions of these materials were measured using a centrifugal precipitation particle size distribution analysis system (SA-CP3L, Shimadzu Corp).
Metabolites were extracted using a methanol : water : chloroform solvent system [ 14], dried using a centrifugal concentrator and stored at −80°C.
An active cooling for PVT system has been maintained by using a centrifugal pump and a radiator have been used in the cycle to dissipate the heat of nanofluid in the environment to maintain proposed inlet temperature.
Au catalysts were deposited on ITO glass substrate using a centrifugal method.
Free AE was removed by repeated ultrafiltration using a centrifugal filter with MWCO (1000 Da).
The supernatants were pooled, dried using a centrifugal vacuum concentrator, resuspended in pure water and filtered.
The mixtures were dried using a centrifugal vacuum evaporator CVE prior to labelling with APTS.
The eluent was evaporated to dryness using a centrifugal concentrator and then lyophilized.
The protein was then concentrated to a range between 10 and 114 mg/mL using a centrifugal concentrator.
Between each step, the supernatants were pooled, and finally concentrated by evaporation using a centrifugal evaporator (SpeedVac).
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