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Radiolabeled PEG 5 -BP-USPIOs can be further PEG 5 -BP-USPIOscentrated to the desired volume using a centrifugal size-exclusion filter with a molecular weight cutoff of 10 kDa (note that around 9% nonsPEG 5 -BP-USPIOsto the filters was found).
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The particle size distributions of these materials were measured using a centrifugal precipitation particle size distribution analysis system (SA-CP3L, Shimadzu Corp).
Porous alumina tubes with homogenous microstructures were prepared from commercial alumina and polymethylmethacrylate (PMMA) powders with two kinds of particle sizes using a centrifugal molding technology.
Free AE was removed by repeated ultrafiltration using a centrifugal filter with MWCO (1000 Da).
The supernatants were pooled, dried using a centrifugal vacuum concentrator, resuspended in pure water and filtered.
The mixtures were dried using a centrifugal vacuum evaporator CVE prior to labelling with APTS.
The eluent was evaporated to dryness using a centrifugal concentrator and then lyophilized.
DOTA- AF SAv prepareDOTA- AF SAvAv and DOTA-NHS ester was readily prepared using a size-exclusion column.
After 30 min reaction time, the immunoconjugates were separated from unreacted BFC using a NAP5 Sephadex size exclusion column and analysed using size exclusion chromatography and ESI-MS.
Unbound biotin-EGF was removed using a size exclusion column.
The aqueous material was concentrated to 50 µl using a YM-30 size exclusion filter.
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