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Between each step, the supernatants were pooled, and finally concentrated by evaporation using a centrifugal evaporator (SpeedVac).
Extracts were evaporated to dryness using a centrifugal evaporator (Heto Maxi Dry Plus) and derivatised using 100 μL N,O-bis trimethylsilyl trifluoroacetamide aN,O-bis trimethylsilyl trifluoroacetamide1]) and 50 μL anhydrous pyridine atrimethylchlorosilane
The solvent layer obtained was evaporated to dryness using a centrifugal evaporator (EYELA, Tokyo, Japan) set at 40 °C, and reconstituted in 5 mL of the mobile phase used for the HPLC MS/MS analyses.
Extracts were evaporated to dryness using a centrifugal evaporator (Heto Maxi dry plus) and derivatised using 100 μL N,O-bis trimethylsilyl trifluoroacetamide aN,O-bis trimethylsilyl trifluoroacetamide1]) and 50 μL anhydrous pyridine atrimethylchlorosilane
The obtained solution was filtered and subsequently dried using a centrifugal evaporator (Genevac Ltd., UK) for 48 h, following evaporated in a rotavapor (IKA laboratory, Germany) at 25°C.
For biomass yield estimations (g dry weight L−1), the contents of 10 unsampled Bioscreen wells were pooled at t124h and dried to constant mass using a centrifugal evaporator (Heto Maxi Dry Plus).
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The mixtures were dried using a centrifugal vacuum evaporator CVE prior to labelling with APTS.
After the sonication, the organic solvent was evaporated rapidly using a rotary evaporator to form carboxylated MNC, and free molecules were removed using a centrifugal filter (Centriprep YM-3, 3,000 Da cutoff, Amicon, Millipore, Billerica, MA, USA)[17].
Lipids were extracted using the method of Bligh and Dyer, dried in a centrifugal evaporator, dissolved in chloroform, and applied to the columns.
Samples were homogenized every hour, then the TFA was evaporated in a centrifugal evaporator at 45°C.
The mixture was then vortex mixed before centrifugation at 3,000 rpm for 10 min, with transfer of the supernatant, using gel-loading tips, to a 13 × 100 culture tube, and then allowing evaporation to dryness in a centrifugal evaporator (∼1 h).
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