Sentence examples for using a cell sorting from inspiring English sources

Recently, an investigation of the transcriptional response of Arabidopsis roots to abiotic stress, including the response to Fe deficiency, has been performed by Dinneny et al. using a cell sorting approach to increase the resolution of the analysis [ 37].

We used a cell sorting strategy, followed by quantitative real-time PCR, to study TOX3 gene expression in the mouse mammary gland.

Using a cell-sorting machine that can separate out the marked cells, the team obtained reproductive cells from mouse ovaries and showed that the cells would generate viable egg cells that could be fertilized and produce embryos.

The toxicity of FTY720 to neutrophils had been examined by fluorescence-activated cell sorting (FACS) using a Cell Apoptosis Detection Kit (BD Biosciences, CA, USA).

After Hoechst 33342 staining of activated T cells and Jurkat cells, cells were sorted using a cell sorter (FACSAria Special Order System Cell Sorter; BD Biosciences).

Cells were sorted using a cell sorter (BD science), collected in 100% FBS, and processed for subsequent total RNA isolation using an RNAqueous-4PCR kit (Ambion, #1914).

The labeled cells were washed and sorted using a cell sorter cytometer (MoFlow, Beckman, Villepinte, France), while discriminating the cells based on their size, granularity and fluorescence (CD3+ and 7AAD−) allowing for the isolation of >95% pure T lymphocytes.

Human CD34+ cells were purified from umbilical cord blood (CB) samples as previously described [ 56, 62]: mononuclear cells were isolated by Ficoll-Hypaque gradient separation, washed twice with PBS, and then CD34+ cells separated using a magnetic cell sorting procedure (EasySep® Human CD34 Positive Selection Kit, StemCell Technologies).

PBMCs collected from 450 mL of blood from donor sheep 4125 (Table 1) were further processed to enrich for B lymphocytes using a magnetic cell sorting system (MACS; Miltenyi Biotech, Auburn, CA).

At least 10,000 events were measured using a fluorescence-activated cell sorting instrument (FACSCalibur; Becton Dickinson, San Jose, CA, USA), and cell flow cytometry data were analyzed using CELLQUEST software (Becton Dickinson).

Here, we established an experimental evolution approach for improving microbial metabolite production by imposing an artificial selective pressure on the fluorescent output of a biosensor using fluorescence-activated cell sorting.