Sentence examples for using a cell sorter from inspiring English sources

Isolated CD8+ T cells were stained with fluorophore-conjugated anti-CD8α, and CD44, CD62L Abs, and naive CD8+ T cells were purified by gating on CD8α+CD44loCD62Lhi using a cell sorter.

Isolated CD4+ T cells were stained with fluorophore-conjugated anti-CD4/CD8α, and CD44, CD62L Abs and naive CD4+ T cells were purified by gating on CD4+/CD8α+CD25−CD44loCD62Lhi cells using a cell sorter.

When this ALDH+ cell population was isolated from A2780DR cells using a cell sorter, ALDH1A1 level in the ALDH+ population was substantially higher than those in the A2780DR and ALDH-negative (ALDH−) cell subpopulations from A2780DR (Fig. 1c).Transcript levels for ALDH1A1 were 83-fold higher in ALDH+ compared to that in ALDH− (Fig. 1d).

As one example, we could build a clinical grade cell sorter so that researchers can do cell therapy using a cell sorter that can get FDA [U.S. Food and Drug Administration] approval rather than the makeshift way we do it now.

All sorting were performed using a cell sorter flow cytometer FACSAria (BD Bioscience, San Jose, CA, USA).

After washing, cells were resuspended, and CD4+ or CD8+ T cells were isolated using a cell sorter separation technology (Beckman coulter).

Donaldson used a cell sorter to separate the cells of each tissue, and introduced hTERT and a mouse oncogene called BMI-1 into the cells to try to immortalize them.

To corroborate our hypothesis, we used a cell sorter to fractionate human sperm samples previously incubated with MT-G.

GFP+, GFP− and Total Sorted tumor cells were sorted into 1 ml of 100% FBS using a MoFlo cell sorter, excluding cell debris, cell doublets, and autofluorescent cells.

CD4−CD8− DN cells were purified by depleting CD4+ cells and CD8+ cells using a magnetic cell sorter (Miltenyi Biotec).

CD4+ T cells were separated into CD4+CD25+ Treg and CD4+CD25- Th cells using a MoFlo cell sorter (Cytomation) and purity of the sorted T cell subsets reached 95-97%.