The cells were then scraped using a cell scraper and the lysate transferred to a 15 mL conical tube.
BV-2 cells were then collected using a cell scraper and stained with APC-Cy7-conjugated anti-mouse CD11b (M1/70, Biolegend).
At 20 h p.p.c., colonized amoebae were detached from the tissue culture flask using a cell scraper, plated onto coated coverslips, and allowed to adhere for 30 min at room temperature.
The cells were dislodged using a cell scraper.
Cells were split every 4 6 days using a cell scraper.
Cells were washed with tris-buffered saline (TBS) and harvested using a cell scraper.
Transfected cells were checked for expression of GFP by fluorescence microscopy and harvested using a cell scraper.
Dissociated colonies were removed from plates using a cell scraper and transferred into six-well low-attachment plates (Corning).
Then, cells were collected using a cell scraper, boiled for 5 minutes and sonicated for 10 seconds.
The Laemmli buffer was added directly to the infected cells and scraped off the tissue culture treated Petri dishes using a cell scraper.
Sheets of cells were washed and scraped off the dish using a cell scraper, cryoprotected in 30% sucrose in PBS and rapidly frozen in OCT (Tissue Tec ®).
