Sentence examples for using a cell lysate from inspiring English sources

The utility of this atmospheric pressure CREMS was demonstrated using a cell lysate fraction from E. coli.

Right tibia bone tissue proteins and in vitro cellular proteins were extracted using a cell lysate buffer described previously [35], [36].

To further substantiate the results, all sera were analysed by the Western blot technique using a cell lysate of PancTu-1 cells (p53 mutation at codon 176) as antigen source.

By immunoblotting analysis using a cell lysate containing overexpressed p53 protein as well as affinity-purified recombinant p53 protein as antigens, we have detected anti-p53 antibodies in 11.2% (61 of 501) of gastric cancer patients, but in none of 46 cancer-free individuals.

For all other forms of PCR, we used a cell lysate (Gloor et al. 1993) modified as follows: ∼1.5 × 10 cells were centrifuged and the culture medium removed.

Cell-free protein synthesis systems often use a cell lysate from E. coli cells, rabbit reticulocytes or wheat germ to supply the protein translation machinery and a recombinant T7 RNA polymerase to couple transcription to translation.

Cells were washed 3 times in cold PBS, mixed, harvested and lysed in Milli-Q water using a Cell Harvester; cell lysate was mixed with scintillation liquid (Optiphase Hisafe2, PerkinElmer, Shelton, CT) and 3H activity was measured in a scintillation counter (Tri-Carb 1600 TR, Packard, Meriden, CT).

The remnant cells were used as a cell lysate.

The cells were lysed using a cell sonicator (SMT), and the lysate was clarified by centrifugation at 15 000 × g for 60 min.

Recently, we produced a tumour antigen-presenting cells (TAPCells) vaccine using a melanoma cell lysate, called TRIMEL, as an antigen source and an activation factor.

A previous study showed that T. gondii CK2-dependent phosphorylation is resistant to a 10 μM concentration of staurosporine using a Toxoplasma cell lysate [ 24].