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The cDNA were synthesized using a cDNA synthesis kit (Bio-Rad Lab, US).
After removing any trace amounts of DNA contamination, the RNA (1 μg) was reverse transcribed into cDNA with oligo-dT as the primer using a cDNA synthesis kit (Bioline, UK) according to the manufacturer's protocol.
Total RNAs of the isolates were extracted using Trizol reagent (Invitrogen, Carlsbad, USA) according to the manufacturer's instruction, and cDNAs were synthesized using a cDNA Synthesis Kit (Takara, Dalian, China), from which hph fragments were amplified with 10 μM primers hphF and hphR (Table 1).
Double-stranded cDNA was prepared using a cDNA Synthesis Kit (Amersham Bioscience, USA).
Total RNA was reverse transcribed into first-strand cDNA using a using a cDNA synthesis kit (GE Healthcare, Stockholm, Sweden).
Double strand cDNA was synthesized from one µg of total RNA using a cDNA Synthesis System (InvitroGen, Basel, Switzerland) with the T7-(T)24 primer.
RNA from DCN pools was extracted using the RNeasy Micro kit (Qiagen) and retrotranscribed using a cDNA synthesis kit (Applied Biosystems).
RNA (10 µg) was reverse transcribed using a cDNA synthesis kit (Roche, Indianapolis, IN) and was diluted to a final volume of 100 µl.
The enriched RNA was converted into double-strand cDNA by using a cDNA synthesis kit (Invitrogen) following the manufacturer's protocol, except for using the biotin-labeled primer based on the 3' end adaptor sequences for the priming (5' biotin-ATCTAGAGCGGCCGCAATGGCCACTCTGCGTTGATAC).
Total RNA was prepared from cultured cells under hypoxia (1% oxygen) for 0, 8, 12 and 24 h RNA was isolated according to the manufacturer's protocol (Qiagen) and reverse transcribed using a cDNA synthesis kit (Quanta BioSciences).
For NBS patients and controls, total RNA was extracted from PBMC using RNeasy RNA purification kits (Qiagen, Germany) and first-strand cDNA synthesis was performed with a CγA primer (5'-GTCCTTGACCAGGCAGCCCAG-3') using a cDNA synthesis kit (Pharmacia, Uppsala, Sweden).
More suggestions(15)
using a radiotracer synthesis
using a network synthesis
using a cdna archive
using a cdna hybridization
using a cdna cloning
using a cdna template
using a cdna macroarray
using a cdna serial
using a novel synthesis
using a cdna array
using a cdna riboprobe
using a narrative synthesis
using a convex synthesis
using a cdna chip
using a cdna platform
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