Sentence examples for using a cdna subtraction from inspiring English sources
Previously, we found several pigmentation genes using a cDNA subtraction and candidate gene approach [ 10, 11, 13- 16].
Using a cDNA subtraction strategy we identified five genes, whose spatial and temporal patterns of transcription suggest involvement in the late phases of spermatogenesis.
Using a cDNA subtraction strategy, five male-specifically or highly male-biased expressed genes were isolated, four of which remain unannotated in the An.
We performed a cDNA subtraction using RNA from uninfected livers because of the high potential for host RNA contamination.
In a previous study, we performed a cDNA subtraction and microarray analysis using the epithelial tissue of induced and non-induced tadpoles and detected cDNA clones that were uniquely up-regulated or down-regulated in the induced and non-induced tadpoles [18].
Two kinds of SSH was performed for the synthesized cDNAs, using a PCR-Select cDNA Subtraction Kit (Clontech), according to the manufacturer's instruction.
Using normalized cDNA subtraction as a transcript profiling tool to identify differentially expressed transcripts, we isolated several genes that may contribute toward understanding the mechanisms involved in meiosis and ovarian follicle differentiation in a mono-ovulatory species.
The cDNAs were subtracted using BD PCR-Select cDNA Subtraction Kit (Clontech) in both forward and reverse directions.
In conclusion, using normalized cDNA subtraction as an alternative and complementary transcript profiling tool to DNA microarrays will help identify novel genes and low abundance transcripts, therefore achieving a more comprehensive global view of the transcriptome in the biological system studied.
Subtracted cDNA library was constructed by using CLONTECH PCR-Select cDNA subtraction kit (CLONTEC H Laboratories Palo Alto, CA) following the method provided by the manufacturer.
The macrophage inhibitory cytokine-1 (MIC-1) gene is a member of transforming growth factor- β (TGF- β) superfamily and was originally isolated from macrophages using the cDNA subtraction method (Bootcov et al, 1997).
