Sentence examples for using a cdna serial from inspiring English sources

A standard curve was generated for each ZIP gene and VvGAPDH (as housekeeping gene) using a cDNA serial dilution.

For each gene, a standard curve was generated using a cDNA serial dilution, and the resultant PCR efficiency calculations (ranging between 1.81 and 2.0) were imported into relative transcript level analysis.

For each gene, a standard curve was generated using a cDNA serial dilution, and the resultant PCR efficiency calculations (ranging between 1.849 and 1.989) were imported into relative expression data analysis.

Actin was selected for normalization due to its consistent transcript levels throughout leaf and fruit tissues, with crossing threshold (Ct) values changing by less than 2. For each gene, a standard curve was generated using a cDNA serial dilution, and the resultant PCR efficiency calculations (ranging between 1.839 and 1.945) were used for relative transcript level analysis.

Primer efficiency was determined using a cDNA pool with serial dilutions (1, 10−1, 10−2 and 10−3).

Standard curves were generated for all primers using WT cDNA serial dilutions and gave amplification efficiencies of 90 100%.

Cycling conditions were 95°C for 20 min, then 40 cycles of 95°C for 1 min and 60°C for 20 min. Expression was determined for duplicate biological replicates and triplicate technical replicates using a serial dilution cDNA standard curve per gene.

For each set of primers (Additional File 8) a standard curve was constructed using a serial dilution of cDNA solution.

The data demonstrate that LDAs are able to accurately and quantitatively detect 2-fold changes in target copy number as was demonstrated using a serial dilution of cDNA.

Primer efficiencies were measured using a serial dilution of stock cDNA at 1 1, 1 10 and 1 100.

qPCR assays were tested initially using a serial dilution of ERCC cDNA and PCR efficiencies calculated (see Additional Data Table 2: MIQE Additional information).