Sentence examples for using a cdna sample from inspiring English sources

The cDNA library was constructed using a cDNA Sample Preparation Kit Catt # RS-930-1001, Inc.mina Inc., San Diego, CA), following the manufacturer's instructions.

Antisense and sense IFN-α cDNAs were synthesized by a standard PCR protocol (Invitrogen Platinum Taq) using a cDNA template from a rat liver sample of acute liver injury (24 h after CCl4 ingestion).

Subsequently, a dilution series was analyzed for each primer pair using a composite cDNA sample from bodies and glands of all stages in order to obtain standard curves for determining primer efficiencies.

Values were obtained from the average of duplicate PCRs run on the same gel and assays were repeated three times for each gene, using a fresh cDNA sample each time.

Relative expression values (REV) were calculated using the standard curve method (User Bulletin 2: ABI PRISM 7700 Sequence Detection System) using serial dilutions of a cDNA sample containing the target sequence [ 70].

For a human control, we used a composite cDNA sample from 10 pooled subjects in this study.

As a control, the expression of gene rp49 [32] was monitored by PCR using the same cDNA sample used for the analysis of yp2.

cDNA was purified using the cDNA Sample Cleanup Module provided with the One-Cycle cDNA Synthesis Kit and resuspended in 14 µl Elution Buffer.

Total RNA was extracted using the Trizol reagent (Sigma, USA) from CNE2 and NP69 according to the manufacturer's instructions and was reverse transcribed into cDNA samples using a Transcriptor First Strand cDNA Synthesis Kit (Roche, Germany, 04 896 866 041).

Real time PCR was performed using a 1 μl cDNA sample as template in a 20 μL reaction on a Step One Plus instrument (Applied Biosystems, Carlsbad, CA).

A standard curve was generated using a "calibrator" cDNA (chosen among the cDNA samples), which was serially diluted and analyzed for all genes to determine the amplification efficiencies of individual genes.