Sentence examples for using a cdna library from inspiring English sources

Here, we demonstrate that the cDNAs encoding the five major structural families of antimicrobial peptides can be elucidated by a single step "shotgun" cloning approach using a cDNA library constructed from the source material of the peptidomic studies the defensive skin secretion itself.

In order to identify proteins that interact with plant transcriptional complexes, we performed a two-hybrid screen in yeast using a cDNA library from embryogenic rice suspension cultures and the plant transcriptional activator viviparous-1 (vp1) as 'bait'bait

To investigate the ligand(s) interacting with TIRC7 we established a yeast two-hybrid screen using a cDNA library of allo-activated human peripheral blood lymphocytes (PBL).

The cDNA fragment of Dg (nucleotide positions 327 826 of the coding sequence of Dg-RA) was amplified by PCR using a cDNA library derived from Drosophila melanogaster.

We show that the long march increases contig length and absolute coverage (compared to the same number of reads produced without the procedure) using a cDNA library generated from Plasmodium falciparum, the protozoan parasite responsible for the most deadly form of human malaria.

Double-stranded cDNA was synthesized by using a cDNA library construction kit (Takara Bio, Shiga, Japan).

Additionally, most previous studies used a cDNA library of one tissue (Table  2), whereas we used a normalised cDNA library comprising multiple tissues and individuals.

The authors used a cDNA library generated from 4T1 cells, a cell line that efficiently colonizes multiple organs, to select for genes that mediate metastases.

Further, traditional transcriptome studies generally use a cDNA library of one tissue, while transcriptome sequencing based on NGS methods can work on a normalized cDNA library comprising multiple tissues and individuals, which would considerably reduce the sequencing cost.

To identify proteins that interact with subolesin in yeast two-hybrid experiments, we used a cDNA library obtained from tick eggs because subolesin is expressed in tick embryos and gene knockdown affects egg development [ 8, 12].

We made a heterologous screen using a cDNA human library as prey and LET-756 as bait.