Sentence examples for using a cdna hybridization from inspiring English sources

In contrast, the PCR-based suppression subtractive hybridization (PCR-SSH) technique is a powerful method that overcomes the problem of differences in mRNA abundance by using a cDNA hybridization procedure that normalizes sequence abundance by kinetic suppression in the PCR subtractive step to afford greater than 103-fold enrichment of rare sequences [20].

Differential gene expression in non-malignant and malignant nasopharyngeal epithelial (NPE) cells, using a cDNA array hybridization method, has evidenced fra-1 expression only in the malignant cells [ 13].

The c- fos mRNA levels were measured by in situ hybridization using a cDNA riboprobe as described elsewhere (39).

Wang et al. (2008) used a cDNA microarray containing 1,920 suppression subtractive hybridization clones to detect transcript profile differences in resistant and susceptible cultivars under controlled BPH feeding (Wang et al. 2008).

Using cDNA hybridization to oligonucleotide microarrays, they reported that the mRNA for genes such as cox3 increase slightly during progression of the infection, whereas our northern analyses clearly indicate that cox3 mRNA decreases quite dramatically.

Norelli et al. [ 11] identified transcripts that are differentially expressed between control and E. amylovora-infected shoots using suppression subtractive cDNA hybridization.

Mechanisms of PM resistance in the Chinese wild species of V. pseudoreticulata and Vitis quinquangularis were investigated using a suppression subtractive hybridization cDNA library that was constructed from E. necator-infected leaves.

To determine the quantity of genomic DNA required to achieve adequate hybridization, three different quantities of genomic DNA, 7.5 (the amount typically used in cDNA hybridizations), 30, and 39 μg, from L. sativa cv.

Cross-species hybridization using a multi-species cDNA microarray is a powerful tool for the discovery of genes involved in evolutionarily conserved molecular mechanisms.

Then, mRNA was purified with a purification kit (Promega) and used for the differentially-expressed first strand cDNA synthesis using a PCR-based subtractive hybridization kit (Clontech) following the protocol supplied by the manufacturer.

The transcript levels of two samples were assayed using both direct cDNA: cDNA hybridization (cDNA1 cDNA2) as well as by hybridizing to genomic DNA (cDNA gDNA).