Sentence examples for using a cdna cloning from inspiring English sources
Using a cDNA cloning and sequencing approach we have shown that F. tularensis expresses homologues of several sRNAs that are well-conserved among diverse bacteria.
Metatranscriptome analysis at a small scale using a cDNA cloning and sequencing approach already demonstrated that the genes and functional categories that are enriched in the small intestine metagenome, were also among the highest expressed functions [ 4].
A calibration standard curve was established using a cDNA clone containing qPCR product targeted by each IGF1 TaqMan assay.
In order to fuse the IQ-tail domain of ATM1 to GFP, we used a cDNA clone kindly provided by Dieter Volkmann (University of Bonn, Germany) and the following primers: Fwd-GGG GTA CCC GTA CTC TCC ACG GCA TT and Rev-CGG GAT CCG TGC TTG GGA ATG CTG CC.
RNA preparation and gene expression profiling using a cDNA microarray containing 44,500 cDNA clones, representing around 30,300 unique genes, has been performed and reported in 90 of these tumours in comparison to 22 non-tumour gastric mucosae [ 14, 15].
Here, we demonstrate that the cDNAs encoding the five major structural families of antimicrobial peptides can be elucidated by a single step "shotgun" cloning approach using a cDNA library constructed from the source material of the peptidomic studies the defensive skin secretion itself.
Furthermore, by expression cloning using a cDNA library of the human MDR fibrosarcoma cell line HT1080/DR4, we identified the antigen of LMR-12.
We sequenced 126 clones using a cDNA library that mimics the generation of GLGI fragments from SAGE tags.
Thus nearly all Arabidopsis genes with expression detectable using current cDNA cloning methods are currently represented by a FL-cDNA sequence.
Using the services of Evrogen [ 50], full length cDNA was constructed using the Smart cDNA cloning methodology [ 51] and normalized using a duplex-specific nuclease [ 50].
Arabidopsis thaliana is inoculated with PPV using different methods: i) mechanically using an inoculum derived from pICPPVnkGFP-infected Nicotiana benthamiana leaves [ 6, 13], ii) by biolistics using a pICPPVnkGFP cDNA clone [ 14] and iii) by agroinoculation with a pBINPPVnkGFP clone introduced in Agrobacterium tumefaciens [ 5].
