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CEW, YL, AL, and BAM performed the microarray analysis using a cDNA chip they produced.
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While SABS treatment did not improve sensitivity for CombiMatrix ElectraSense® arrays using purified influenza A cDNA, chip sensitivity was improved 10-fold for randomly amplified targets.
A pool of 10 normal and 10 tumour samples was hybridised to a cDNA chip containing 10 000 cDNA sequences.
We previously established a genomic profile of human MSCs before and after their differentiation into chondrocytes, using the cDNA chip technology (F Djouad, D Noël, unpublished data).
The direct labeling procedure was used with 1 μg of total RNA sample; double-stranded cDNA was synthesized with a T7 promoter-containing oligo (dT) primer using a Gene chip one-cycle cDNA synthesis kit (Affymetrix), followed by in vitro transcription using a Gene chip IVT labeling kit (Affymetrix).
The Agilent 2100 Bioanalyser (Agilent Technologies) was used to assess the purity of the ds cDNA libraries using a DNA chip protocol.
This is the first report of plant virus detection by using cDNA chip technology.
11 A relatively new method of genetic research is to study expression of genes in specific tissues by microarray analysis using an RNA or cDNA chip.
cDNA libraries were validated using a High Sensitivity Chip on the Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA).
cDNA libraries were validated using a High Sensitivity Chip on the Agilent2100 Bioanalyzer™ (Agilent Technologies, CA).
Using potato cDNA chips, it has been shown that infections of ToRSV and PPV result in the suppression of 34% (83/243) of chloroplast-related genes, while no common genes are among the induced genes in ToRSV- and PPV-infected N. benthamiana.
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