In a previous study, we have analysed the expression profile of apoptosis-regulating genes using a cDNA-based microarray and found overexpression of the antiapoptotic bcl-2 family member, bfl-1, in B-CLL cells with an apoptosis-resistant phenotype.
We first identified a study, in which the gene expression profiles of 35 human tissue types were also established using a cDNA-based microarray experiment with a common reference design [7].
For example, changes in gene expression induced by gibberellic acid (GA) in the developing xylem was studied using a cDNA-based microarry analysis [ 15].
Reasoning that genes controlling differences in dispersal and host-selection behavior are likely to be expressed in the brain, we therefore searched for gene expression differences in heads of female beetles using a cDNA-based microarray.
Our whole genome analysis identified a large set of genes (987 IDs) specifically regulated in the sen1 mutant nodules and confirms the majority of the previous observations by Suganuma and co-workers, who identified a total of 93 genes differentially regulated in this mutant compared to wild-type using a cDNA macroarray based on approximately 18,000 nonredundant clones [29].
For the ecdysone plus heat shock experiments, a cDNA based microarray was used and the microarray experiments and analysis were carried out following the methods of Neal and co-workers [68].
So far there has been no report focusing on differences of genetic alterations between subtypes of thymoma using a cDNA microarray based-CGH method (microarray-CGH).
The gene expression profile of the sub-group of AR-V high bone metastases was compared to the profile of other CRPC bone metastases, by using an Illumina based cDNA array including 48803 gene probes.
We recently cloned a putative rd cDNA, zr.408, using a strategy based on subtractive and differential hybridization.
In a first step, overexpressed cDNA clones were isolated using a PCR based subtractive cloning method.
Two rounds of B chromosome-specific cDNA selection were conducted using a method based on previously published selection of hybrids by affinity capture (SHAC) [ 29].
