Total RNA was isolated using Absolutely RNA Miniprep Kit (Stratagene, La Jolla, CA, USA) and reverse transcribed to cDNA on a PCR System 2400 (PerkinElmer) using a High Capacity cDNA Archive Kit (Applied Biosystems).
Five micrograms of total RNA from each cell line profiled by Affymetrix microarrays was converted to cDNA using an ABI High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA).
Five hundred nanograms of total RNA were used to synthesize cDNA using a High-Capacity cDNA Archive Kit.
For reverse transcription, 5 µl of RNA sample with 45 µl of RNase-free water was used to synthesize cDNA using a High-Capacity cDNA Archive Kit (Applied Biosystem, Foster City, CA).
RNA was reverse-transcribed into cDNA using a High-Capacity cDNA Archive kit (Applied Biosystems, Foster City, CA, USA) and random primers.
Five micrograms of each sample were reverse transcribed into cDNA using a high-capacity cDNA archive kit (Applied Biosystems).
Extracted total RNA was reverse-transcribed into single-stranded cDNA using a High Capacity cDNA Archive Kit (Applied Biosystems, Foster City, CA, USA) and a TaqMan microRNA reverse transcription kit (Applied Biosystems).
For qPCR analysis, 1 μg of total RNA was reverse-transcribed to cDNA using a high-capacity cDNA archive kit (Applied Biosystems, Life Technologies, Paisley, UK).
The extracted total RNA was reverse-transcribed into single-stranded cDNA using a High-Capacity cDNA Archive Kit (Applied Biosystems, Warrington, UK).
