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VTG was measured in the plasma of all fish using a carp VTG ELISA validated for use with fathead minnows (Tyler et al. 1999).
Although adding the zebrafish intermediary step (SRBH) improved ortholog assignment by 8%, 581 assignments that failed using this procedure were successfully found using a carp vs. human RBH alone (figure 1, route 1).
Quantification of whole-body VTG was determined using a carp VTG enzyme-linked immunosorbent assay (ELISA) (Tyler et al. 1999) until assayed in the VTG ELISA that has been validated for use in the roach (Tyler et al. 1996).
At the end of the 14-day exposure period, the fish were sacrificed using a lethal dose of anesthetic, blood was sampled via cardiac puncture, and plasma samples were analyzed for VTG concentrations, using a carp enzyme-linked immunosorbent assay (ELISA) (Tyler et al. 1999).
We used immunohistochemistry to detect the presence and assess the distribution of VTG in the body tissues of 10 male and 10 female juvenile roach from the control and 80% effluent treatments, using a carp VTG polyclonal antibody (validated for use in the roach; Tyler et al. 1996).
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Plasma VTG concentrations were determined using a carp-VTG enzyme-linked immunosorbent assay (ELISA) validated for the measurement of VTG in fathead minnows (Tyler et al. 1996).
VTG was quantified in whole-body extracts in ELS fish and in plasma in adult fish using a carp-VTG enzyme-linked immunosorbant assay (ELISA) that has been validated for use in the roach (Tyler et al. 1999).
Hence to bridge this gap the decontaminated effluents were subjected to fish bioassay analysis using a major carp (Labeo rohita) as bioindicator.
Enrofloxacin solution (60 g/L) was forced into the stomach of the grass carp using a 1-ml syringe fitted with an obtuse 7# gauge needle.
The aim of the present study was to describe lethal and sublethal effects of terbuthylazine-2-hydroxy on embryo and larvae of common carp using a 35-day embryo larval toxicity test.
Quantification of VTG in the plasma samples was achieved using an established homologous carp enzyme-linked immunosorbent assay (Tyler et al. 1999) that has been validated for use with a wide variety of cyprinid fish (Tyler et al. 1996).
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