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Serum leptin and adiponectin levels were measured according to the manufacturer's protocol using a canine leptin commercial sandwich enzyme-linked immunosorbent assay (ELISA) kit (Millipore, Billerica, MA, USA) and canine adiponectin commercial ELISA kit (Milipore, Billerica, MA, USA).
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We need to start from scratch using a canine model".
To evaluate repelling magnetic implants to treat obstructive sleep apnea (OSA) using a canine model.
Using a canine to meet needs represented healthier coping than using drugs or alcohol or engaging in other destructive behavior.
This was shown by Freeman and later tested by Whiteside et al using a canine model.
All experiments used a canine model of HCA developed in by the Baumgartner laboratory.
Plasma leptin concentrations were measured using a mouse leptin ELISA kit (Crystal Chemicals, Chicago, USA).
The levels of leptin, adiponectin were measured using a mouse leptin ELISA kit (Morinaga, Yokohama, Japan), a mouse adiponectin ELISA kit (Ootuka, Tokyo, Japan), respectively.
To further examine the interaction between mutant V145E leptin and leptin receptor, coimmunoprecipitation analysis was performed from brain using a leptin receptor antibody.
Sixty-three compounds were tested using a hASBT Madin Darby canine kidney cell monolayer model.
Samples were assayed in triplicate (200 µl) using a rabbit anti-ovine leptin primary antibody, iodinated ovine leptin and sheep anti-rabbit secondary antibody.
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