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The ESI-Q-ToF was calibrated using a calibration standard (M600, Applied Biosystems) and the measuring accuracy was determined to be ±0.05.
The counts in this region are converted to mCi using a calibration standard that is loaded into the software.
Calibration accuracy was verified for each batch using a calibration standard solution with labeled and native analytes.
Individual FAEEs were identified via their unique retention times and confirmed using a calibration standard composed of all FAEEs of interest (1,000 μg/ml; Cayman Chemicals, Ann Arbor, MI).
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Instrument response was evaluated initially, after every 10 samples, and at the end of each analytical run using a calibration verification standard and blank.
Apparent molecular weights were determined using a calibration plot with standard proteins as a reference (broad range SDS-PAGE Standards, Bio-Rad Laboratories). Bio-Rad Laboratories
The concentration of BG in each sample was automatically calculated using a calibration curve with standard solutions ranging from 31.25 to 500 pg/mL.
The molecular weight was then determined based on polystyrene which was used as a calibration standard.
Solutions of potassium thiocyanate were used as a calibration standard.
Gallic acid solutions were used for a calibration standard curve.
Recombinant human sFasL and VEGF165 (R & D, Minneapolis, MN, USA) were used as a calibration standard.
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