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The mean % recoveries were calculated using a calibration graph.
The concentration was measured with the help of a spectrophotometer Spectrometer Lambda 35 UV/Vis Perkin Elmer Instruments at λ = 480 nm using a calibration graph.
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Calibration of the prognostic model was determined using a calibration curve.
38 Model calibration was first explored using a calibration plot.
The calibration is illustrated using a calibration plot.
An aliquot (0.01 mL) from each sample was separately analyzed on a high-performance liquid chromatography (HPLC) (Ultimate 3000, Dionex) coupled to a hybrid triple quadrupole/linear ion trap mass spectrometer (3200 Q TRAP, Applied Biosystems) using a multilevel calibration graph with [H]-labeled internal standards as described before [ 57, 58].
Using a calibration-function method35, the pollen data were transferred quantitatively into annual precipitation (PANN).
Then their first derivative spectra (D1) were recorded using scaling factor = 10 and ∆λ = 8 and a calibration graph was constructed relating the amplitude of the obtained (D1) spectra of PAP at 314.5 nm versus the corresponding concentrations.
The first derivative of the above ratio spectra was then recorded using scaling factor = 1 and ∆λ = 8 and a calibration graph between the amplitude at 219 nm versus the corresponding concentrations of CAF was constructed.
The particulate deposition data was plotted onto a calibration graph (insert deposition against corresponding Borgwaldt RM20S smoke dilution) using GraphPad Prism version 5.02.
a Calibration graph.
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