Sentence examples for using a brief trypsinization from inspiring English sources

The cells were seeded at a density of ~5 × 10 per well using a brief trypsinization.

Using a brief questionnaire, we collected demographic data on participants.

Recent life-threatening events, using a brief validated checklist [ 66].

At time of passage, the majority of stromal cells are collected separately by a brief trypsinization.

After 48 h treatment, culture supernatants which contained floating dying and apoptotic cells were collected, and then merged with the adherent cells which were rinsed with phosphate buffered saline (PBS) and harvested by a brief trypsinization.

Briefly, the cells were detached by a brief trypsinization, isolated by centrifugation, washed in PBS, resuspended in the Cytosol Extraction Buffer containing a mix of DTT and protease inhibitors, homogenized in an ice-cold tissue grinderm and centrifuged at 700 × g for 10 min at 4°C.

Schwann cells were plated into 96-well plates, using brief trypsinization, 24 h prior to experimental treatment.

Exponentially growing cells were passaged twice a week using a standard trypsinization procedure.

On reaching confluence, melanocytes were passaged using a standard trypsinization protocol, and new colonies were seeded at approximately 5000 cells/cm.

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Cells were allowed to grow for the indicated time and then counted in duplicate after trypsinization using a Coulter counter (Fullerton, CA).