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The circular MCM complex is loaded onto dsDNA using a break in the MCM ring between Mcm5p and Mcm2p (Bochman and Schwacha 2007) by the actions of Ctd1 and Cdc6p (Randell et al. 2006; Remus et al. 2009).
Park further from the door.Try using a break room or washroom on a different floor, or walking the long way around to get to the printer or copier.
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Thus, many in my generation knew they were using a broken (but legal) system to shirk their duty.
We optimized the program for our data using a break-point threshold of -0.0013 and a coefficient of variation of 0.045.
The advantages of using a break-part FISH probe for gene translocation are that it can detect the translocations irrespective of fusion partners.
The MYC gene copy number was assessed by FISH using a break-apart probe (Abbott Molecular, Abbott Park, IL, USA) mixed with a single-colour (aqua) MYB probe (chromosome 6q23) as an internal control.
TMPRRS2-ERG gene fusion status was determined for Cambridge RP samples using a break-apart FISH assay on purpose-made tissue microarrays as previously described (Clark et al., 2008) from tissue cores adjacent to those included in the genomics study — see below.
The emus could use a break.
Standard & Poor's could use a break.
(We can use a break).
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CEO of Professional Science Editing for Scientists @ prosciediting.com