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A phylogenetic analysis of NPR1 proteins using a bootstrap consensus for maximum likelihood (ML) revealed that NPR1 homologs can be classified into three major groups, NPR1, NPR3, and BOP, with high confidence in which both dicot and monocot species have paralogs in each major group (Figure 2).
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Within each heuristic search, 500 discrete starting trees were used and a bootstrap consensus tree was estimated from the resulting trees.
We calculated power using a bootstrap approach.
Using the Neighbour-Joining method, a bootstrap consensus tree was used to represent the evolutionary history of the ORFV011 [ 35, 36].
100 bootstrap replicates were performed to test MPT robustness, the strict consensus of which is shown in Figure 2. A bootstrap consensus tree using only sequences with no stop codons yielded 2 trees.
A bootstrap consensus tree was constructed using the neighbor joining method (Saitou and Nei 1987) with all the Classic OBPs from the three mosquito genomes and the D. melanogaster with 1000 bootstrap replicates (fig. 4).
Trees in the pooled file were summarized using the majority rule consensus method to derive a bootstrap consensus phylogenomic tree.
The evolutionary history was inferred using the neighbor-joining method [ 45] and a bootstrap consensus tree estimated from 1000 replicates [ 46].
A bootstrap consensus tree was built by using a neighbor-joining method packaged in MEGA version 4.0 [ 39].
Phylogenetic trees were inferred on PhyML (phylogenetic estimation using maximum likelihood) (Guindon et al. 2010), and a bootstrap consensus tree was created (1,000 bootstrap replicates, initial tree BioNJ, model of nucleotides substitution GTR [general time reversible]).
Using the filtered 46,237 marker set for the 52 genotypes, a bootstrap consensus tree was constructed.
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