Body fat percent was assessed using a bioimpedance analyzer (Omron HBF-306C).
This variable is straightforwardly measured using a bioimpedance spectroscopy (BIS) device.
The amounts of body fat and fat-free mass were determined using a bioimpedance analyzer (model BIA 310) according to the manufacturer's protocol.
Body composition will be estimated using a bioimpedance analyser, the Tanita MC 180 MA Multi-Frequency Body Composition Analyzer (Tanita Corp ,Tokyo, Japan).
A Maltron Bio-Scan 916 (Maltron International Ltd ,Rayleigh, UK) was used for measurement of body composition and it uses a bioimpedance analysis method.
Subsequently, the volunteers rested on a bed for 5 min before body measurements were taken using the Bodystat®1500 bioimpedance machine (Bodystat Limited).
I sleep using a breathing machine.
Analysis was performed using a BD LSRII machine.
X-ray images were acquired using a microradiography machine (Faxitron Corporation).
Ligations were performed overnight using a PCR machine.
