At 72 h, 1 µCi of 3H-thymidine was added to each well, and the cells were harvested at the end of the culture period and analyzed for 3H-thymidine incorporation using a beta-scintillation counter.
AEG estimated LER and LED using a beta function.
After incubation, the cellular and aqueous phases were separated and incorporation was determined using a Topcount Microplate Beta Scintillation counter (Canberra Packard, Meriden, CO, USA) following the addition of Microscint20 scintillation fluid (Perkin Elmer, Boston, MA, USA).
The H-Thymidine uptake was measured using a LKB beta scintillation counter.
The amount of radioactivity incorporated was determined by using a liquid scintillation beta counter (Beckman Instruments, Inc.Madrid, Spain).
The amount of radioactivity incorporated was determined by using a liquid scintillation beta counter (Beckman Instruments, Inc .. Results shown are the mean [3H]-thymidine incorporation of triplicate wells.
Alternatively, TTR incubated with A-Beta (1 42) and A-Beta alone, were run on a SDS-PAGE gel, transferred to nitrocellulose membranes, and A-Beta immunodetected using an anti-A-Beta antibody (BAM-10, Sigma).
The cells were harvested in semi-automated harvester and the cpm was measured in a beta-scintillation counter.
CTLL-2 cells were harvested onto glass-fiber filters and incorporated radioactivity was counted in a beta-scintillation counter.
Scintillation fluid (Perkin Elmer) was added prior to measuring beta emission (photon counts per minute) using a liquid scintillation counter (Perkin Elmer).
Expression values were normalized using an endogenous beta-actin.
