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The size of pulverised nanoparticles can also be controlled when an automated milling device (wet grinding using a bead mill) is employed27.
Endoh, S., Maru, J., Uchida, K., Yamamoto, K. & Nakanishi, J. Preparing samples for fullerene C60 hazard tests: Stable dispersion of fullerene crystals in water using a bead mill.
Eighteen tomato leaf discs (diameter 10mm) from six individual plants per M3 family were collected in 96-well plates containing 2 steel beads (4mm) per well, and tissues were ground using a bead mill.
Sixteen melon leaf discs (diameter 10 mm) from eight individual plants per M2 family were collected in 96-well plates containing 2 steel beads (4 mm) per well, and tissues were ground using a bead mill.
Liver tissue (≈3 mg) was homogenized by using a bead mill.
A simple protein extraction protocol was established, using a bead mill for homogenizing fly specimens and the denaturing conditions of 6 M urea for efficient solubilization of proteins.
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As anticipated, significant variation was found in the amount of total RNA that could be extracted from the different tissues, the more fibrous heart yielding the lowest average concentration despite our having used a bead mill.
The DMSO-preserved samples were washed twice with DNAB buffer (0.4 M NaCl, 50 mM EDTA), and the tissue slurry homogenized with glass beads (BIO101, USA) using a MagnaLyser bead mill (Roche Diagnostics, Australia) at 3000 rpm for 90 s.
After adding 400 µl RLT buffer (Qiagen, Hilden, Germany), the material was homogenized using a TissueLyser bead mill (Qiagen) and extracted using the BioSprint DNA Plant Kit and the BioSprint 96 workstation from Qiagen.
Tissue pools were homogenized by using a Precellys bead mill homogenizer (Bertin Technologies, Ann Arbor, MI, USA), and DNA was extracted by using the Wizard Genomic DNA Purification Kit (Promega, Southampton, UK) according to manufacturer's instructions.
A bead mill homogenizer (Bullet Blender, Next Advance) was used to lyse the pulverized DFC tissue samples.
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