Genomic DNA was extracted from wound samples using a bead-beating and enzymatic lysis protocol, followed by purification using a QIAamp DNA Mini Kit (Qiagen, USA).
Genomic DNA was extracted from swab samples using a bead-beating and enzymatic lysis protocol, followed by purification using a QIAamp DNA Mini Kit (Qiagen, Valencia, USA).
The DNA for the microbiomes was isolated from the Sterivex filters by removing the filter membranes and performing DNA extractions using a bead-beating protocol (MoBio, Carlsbad CA).
Total DNA of the biofilms samples was extracted and purified using a bead beating method (FastDNATMSPIN Kit for Soil, Bio101 Inc., USA) following the manufacturer's instructions.
Genomic DNA was isolated from M. faveolata sperm using a modified bead-beat protocol.
DNA was extracted from each sample directly using a modified bead beating and solvent extraction protocol [12].
Genomic DNA was extracted from fecal samples using a repeated bead beating procedure (Salonen et al., 2010) and sequenced on Illumina HiSeq 2000.
Thus, we first isolated them using a glass bead beating procedure (see Materials and Methods) inspired from previously published methods [ 26, 27].
Use a bead as your anchor.
Use a bead board to create the pattern before threading.
Bead-beating was performed for 1 min at 2100 rpm using a BioSpec Mini-Bead Beater-96.
